• Detection of environmental DNA from amphibians in Northern Europe applied in citizen science 

      Knudsen, Steen Wilhelm; Hesselsøe, Martin; Rytter, Maria; Lillemark, Marie Rathcke; Tøttrup, Anders P.; Rahbek, Carsten; Sheard, Julie Koch; Thomsen, Philip Francis; Agersnap, Sune; Mortensen, Peter B.; Møller, Peter Rask (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-25)
      Many species of amphibians in Northern Europe are threatened and the local distributions are rarely described in detail. Application of modern molecular methods provides an important supplementary tool for monitoring the distribution and diversity of amphibians. For this purpose, we designed, tested, validated, and optimized 14 species-specific assays on genomic DNA extracted from tissue samples to ...
    • Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic 

      Knudsen, Steen Wilhelm; Hesselsøe, Martin; Thaulow, Jens; Agersnap, Sune; Hansen, Brian Klitgaard; Jacobsen, Magnus Wulff; Bekkevold, Dorte; Jensen, Søren K.S.; Møller, Peter Daniel Rask; Andersen, Jesper Harbo (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-14)
      Monitoring the distribution of marine non-indigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) from marine species all non-indigenous to Europe. The species include sturgeons, a Pacific red algae, oyster thief, a freshwater hydroid from the Black Sea, Chinese mitten crab, ...
    • A National Scale “BioBlitz” Using Citizen Science and eDNA Metabarcoding for Monitoring Coastal Marine Fish 

      Agersnap, Sune; Sigsgaard, Eva Egelyng; Jensen, Mads Reinholdt; Ávila, Marcelo De Paula; Carl, Henrik; Møller, Peter Rask; Krøs, Simon Leed; Knudsen, Steen Wilhelm; Wisz, Mary S.; Thomsen, Philip Francis (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-28)
      Marine biodiversity is threatened by human activities. To understand the changes happening in aquatic ecosystems and to inform management, detailed, synoptic monitoring of biodiversity across large spatial extents is needed. Such monitoring is challenging due to the time, cost, and specialized skills that this typically requires. In an unprecedented study, we combined citizen science with eDNA ...
    • Short-term temporal variation of coastal marine eDNA 

      Jensen, Mads Reinholdt; Sigsgaard, Eva Egelyng; Ávila, Marcelo De Paula; Agersnap, Sune; Brenner-Larsen, William; Sengupta, Mita Eva; Xing, Yingchun; Krag, Marcus Anders; Knudsen, Steen Wilhelm; Carl, Henrik; Møller, Peter Daniel Rask; Thomsen, Philip Francis (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-23)
      Temporal variation in eDNA signals is increasingly explored for understanding community ecology in aquatic habitats. Seasonal changes have been addressed using eDNA sampling, but very little is known regarding short-term temporal variation that spans hours to days. To address this, we filtered marine water samples from a single coastal site in Denmark every hour for 32 h. We used metabarcoding to ...